Once you have covered the main functionality in ggseg
you will want to use it to plot the results of your data. In order to do
this, your data must adhere to certain specifications, so that
ggseg
can manage to merge your data with the atlas you are
using. This means you need to be able to inspect and locate the way the
regions you are working with are names in the internal atlas files. This
vignette should provide the tools you need to figure these features out,
and to manipulate your data to fit these requirements.
There are several ways you can inspect what the data in the atlas
looks like. While each atlas has some small differences, they all share
six main columns:
1. .long - x-axis
2. .lat - y-axis
3. .region - name of region/network
4. .hemi - hemisphere (left or right)
5. .side - side of view (medial, lateral, sagittal or
axial)
Most atlases also have a label
column, which are raw
names assigned from the program run to segment/extract data. TO inspect
the atlases, call them in the console.
library(ggseg)
library(dplyr)
##
## Attaching package: 'dplyr'
## The following objects are masked from 'package:stats':
##
## filter, lag
## The following objects are masked from 'package:base':
##
## intersect, setdiff, setequal, union
library(ggplot2)
dk
## # dk cortical brain atlas
## regions: 35
## hemispheres: left, right
## side views: lateral, medial
## palette: yes
## use: ggplot() + geom_brain()
## ----
## hemi side region label roi
## <chr> <chr> <chr> <chr> <chr>
## 1 left lateral bankssts lh_bankssts 0002
## 2 left lateral caudal middle frontal lh_caudalmiddlefrontal 0004
## 3 left lateral fusiform lh_fusiform 0008
## 4 left lateral inferior parietal lh_inferiorparietal 0009
## 5 left lateral inferior temporal lh_inferiortemporal 0010
## 6 left lateral lateral occipital lh_lateraloccipital 0012
## 7 left lateral lateral orbitofrontal lh_lateralorbitofrontal 0013
## 8 left lateral middle temporal lh_middletemporal 0016
## 9 left lateral pars opercularis lh_parsopercularis 0019
## 10 left lateral pars orbitalis lh_parsorbitalis 0020
## # … with 76 more rows
Further inspection of the atlas data can be explored by turning them into tibbles (or data.frames)
as_tibble(dk)
## # A tibble: 90 × 8
## atlas type hemi side region label roi geometry
## <chr> <chr> <chr> <chr> <chr> <chr> <chr> <MULTIPOLYGON>
## 1 dk cortical left lateral <NA> <NA> 0001 (((84.32563 34.46407, 84…
## 2 dk cortical left lateral bankssts lh_b… 0002 (((214.8215 108.8139, 21…
## 3 dk cortical left lateral caudal mi… lh_c… 0004 (((106.16 184.3144, 93.6…
## 4 dk cortical left lateral fusiform lh_f… 0008 (((256.5481 48.35713, 24…
## 5 dk cortical left lateral inferior … lh_i… 0009 (((218.4373 161.6233, 21…
## 6 dk cortical left lateral inferior … lh_i… 0010 (((250.7745 70.75764, 24…
## 7 dk cortical left lateral lateral o… lh_l… 0012 (((277.4615 115.0523, 27…
## 8 dk cortical left lateral lateral o… lh_l… 0013 (((66.26648 69.56474, 56…
## 9 dk cortical left lateral middle te… lh_m… 0016 (((238.0128 91.25816, 23…
## 10 dk cortical left lateral pars oper… lh_p… 0019 (((79.03391 126.496, 74.…
## # … with 80 more rows
Here you can see information about the dk
atlas, and the
main attributes of this atlas. If you want to use external data with
your ggseg
plot, you will need to make sure that your data
has at least one column corresponding in name and content with another
in the atlas you are using.
For instance, here we make some data for the “default” and “visual”
networks in the dk
atlas, and two p values for those two
networks.
= tibble(
someData region=c("superior temporal","precentral", "lateral orbitofrontal"),
p=c(.03,.6, .05)
) someData
## # A tibble: 3 × 2
## region p
## <chr> <dbl>
## 1 superior temporal 0.03
## 2 precentral 0.6
## 3 lateral orbitofrontal 0.05
Notice you we have spelled both the column name and the region names
exactly as they appear in the data. This is necessary
for the merging within the ggseg
function to work properly.
This merge can be attempted before supplying the data to
ggseg
to see if there are any errors.
%>%
dk as_tibble() %>%
left_join(someData)
## Joining, by = "region"
## # A tibble: 90 × 9
## atlas type hemi side region label roi geometry p
## <chr> <chr> <chr> <chr> <chr> <chr> <chr> <MULTIPOLYGON> <dbl>
## 1 dk cortical left late… <NA> <NA> 0001 (((84.32563 34.46407, 84… NA
## 2 dk cortical left late… banks… lh_b… 0002 (((214.8215 108.8139, 21… NA
## 3 dk cortical left late… cauda… lh_c… 0004 (((106.16 184.3144, 93.6… NA
## 4 dk cortical left late… fusif… lh_f… 0008 (((256.5481 48.35713, 24… NA
## 5 dk cortical left late… infer… lh_i… 0009 (((218.4373 161.6233, 21… NA
## 6 dk cortical left late… infer… lh_i… 0010 (((250.7745 70.75764, 24… NA
## 7 dk cortical left late… later… lh_l… 0012 (((277.4615 115.0523, 27… NA
## 8 dk cortical left late… later… lh_l… 0013 (((66.26648 69.56474, 56… 0.05
## 9 dk cortical left late… middl… lh_m… 0016 (((238.0128 91.25816, 23… NA
## 10 dk cortical left late… pars … lh_p… 0019 (((79.03391 126.496, 74.… NA
## # … with 80 more rows
No errors! Yes, the p
column is seemingly full of
NA
s, but that is just because the top of the data is the
somatomotor network, which we did not supply any p values for, so it has
been populated with NA
s. We can sort the data differently,
so we can see the p
has been added correctly.
%>%
dk as_tibble() %>%
left_join(someData) %>%
arrange(p)
## Joining, by = "region"
## # A tibble: 90 × 9
## atlas type hemi side region label roi geometry p
## <chr> <chr> <chr> <chr> <chr> <chr> <chr> <MULTIPOLYGON> <dbl>
## 1 dk cortical left late… super… lh_s… 0031 (((207.634 112.8177, 195… 0.03
## 2 dk cortical right late… super… rh_s… 0031 (((1201.097 114.4479, 12… 0.03
## 3 dk cortical left late… later… lh_l… 0013 (((66.26648 69.56474, 56… 0.05
## 4 dk cortical left medi… later… lh_l… 0013 (((571.041 20.11423, 571… 0.05
## 5 dk cortical right medi… later… rh_l… 0013 (((770.9836 30.46747, 77… 0.05
## 6 dk cortical right late… later… rh_l… 0013 (((1321.97 75.4817, 1320… 0.05
## 7 dk cortical left late… prece… lh_p… 0025 (((157.1559 204.0537, 13… 0.6
## 8 dk cortical left medi… prece… lh_p… 0025 (((476.8878 196.7183, 48… 0.6
## 9 dk cortical right medi… prece… rh_p… 0025 (((923.7245 201.5463, 92… 0.6
## 10 dk cortical right late… prece… rh_p… 0025 (((1226.19 203.939, 1230… 0.6
## # … with 80 more rows
If you need your data to be matched on several columns, the approach is the same. Add the column you want to match on, with the exact same name, and make sure it’s content matches the content of the same column in the data.
$hemi = rep("left", nrow(someData))
someData someData
## # A tibble: 3 × 3
## region p hemi
## <chr> <dbl> <chr>
## 1 superior temporal 0.03 left
## 2 precentral 0.6 left
## 3 lateral orbitofrontal 0.05 left
%>%
dk as_tibble() %>%
left_join(someData) %>%
arrange(p)
## Joining, by = c("hemi", "region")
## # A tibble: 90 × 9
## atlas type hemi side region label roi geometry p
## <chr> <chr> <chr> <chr> <chr> <chr> <chr> <MULTIPOLYGON> <dbl>
## 1 dk cortical left late… super… lh_s… 0031 (((207.634 112.8177, 195… 0.03
## 2 dk cortical left late… later… lh_l… 0013 (((66.26648 69.56474, 56… 0.05
## 3 dk cortical left medi… later… lh_l… 0013 (((571.041 20.11423, 571… 0.05
## 4 dk cortical left late… prece… lh_p… 0025 (((157.1559 204.0537, 13… 0.6
## 5 dk cortical left medi… prece… lh_p… 0025 (((476.8878 196.7183, 48… 0.6
## 6 dk cortical left late… <NA> <NA> 0001 (((84.32563 34.46407, 84… NA
## 7 dk cortical left late… banks… lh_b… 0002 (((214.8215 108.8139, 21… NA
## 8 dk cortical left late… cauda… lh_c… 0004 (((106.16 184.3144, 93.6… NA
## 9 dk cortical left late… fusif… lh_f… 0008 (((256.5481 48.35713, 24… NA
## 10 dk cortical left late… infer… lh_i… 0009 (((218.4373 161.6233, 21… NA
## # … with 80 more rows
Notice how the message now states that it is joining
by = c("region", "hemi")
. The merge function has recognized
that there are two equally named columns, and assumes (in this case
correctly) that these are equivalent.
Notice that everything is case-sensitive, so writing
Region
or Left
will not result in
matching.
ggseg
When you have managed to create data that merges nicely with the atlas, you can go ahead and supply it to the function.
ggplot(someData) +geom_brain( atlas=dk, mapping=aes(fill=p))
## merging atlas and data by 'region', 'hemi'
You can actually also supply it directly as an atlas. For instance,
if you had saved the merged data from the previous steps, you can supply
this directly to the atlas
option.
= dk %>%
newAtlas as_tibble() %>%
left_join(someData) %>%
as_brain_atlas()
## Joining, by = c("hemi", "region")
ggplot() +
geom_brain(atlas = newAtlas,
mapping = aes(fill=p),
position = position_brain(hemi ~ side)
)
It is this possibility of supplying a custom atlas that gives you particular flexibility, though a little tricky to begin with. Lets do a recap of the unwanted results:
= data.frame(
someData region = rep(c("transverse temporal", "insula",
"precentral","superior parietal"),2),
p = sample(seq(0,.5,.001), 8),
AgeG = c(rep("Young",4), rep("Old",4)),
stringsAsFactors = FALSE)
ggplot(someData) +
geom_brain(atlas = dk,
colour="white",
mapping=aes(fill=p)) +
facet_wrap(~AgeG, ncol=1) +
theme(legend.position = "bottom")
## merging atlas and data by 'region'
See how you have three facets, when you only have 2 groups, and that
the “background” brain is not printed in your two groups. This is
because for ggplot, that is what the data looks like. For this to work,
you can supply already grouped data to ggseg
, but you must
make sure they are grouped by the columns you will use for faceting, or
else it will not work.
# If you group_by the columns you will facet by, this will work well.
= someData %>%
someData group_by(AgeG)
# We can now supply the newAtlas as an atlas to ggseg
ggplot(someData) +
geom_brain(atlas=dk,
colour="white",
mapping=aes(fill=p)) +
facet_wrap(~AgeG, ncol=1) +
theme(legend.position = "bottom") +
scale_fill_gradientn(colours = c("royalblue","firebrick","goldenrod"),na.value="grey")
## merging atlas and data by 'region'
This whole procedure can be piped together, so you don’t have to save all the intermediate steps.
%>%
someData group_by(AgeG) %>%
ggplot() +
geom_brain(atlas=dk,
colour="white",
mapping=aes(fill=p)) +
facet_wrap(~AgeG, ncol=1) +
theme(legend.position = "bottom") +
scale_fill_gradientn(colours = c("royalblue","firebrick","goldenrod"),na.value="grey")
## merging atlas and data by 'region'